Multiple recombinase polymerase amplification and low-cost array technology for the screening of genetically modified organisms

The identification of different transgenic products that are potentially present in foods is a priority given their impact on environmental safeness and health care. In this context, reliable, fast inexpensive detection methods demanded to screen the compliance product labelling traceability regulations. We herein developed method combines recombinase polymerase amplification (RPA) multiple format hybridisation assay. This system was optimised for simultaneous amplification/detection 35S promoter, NOS terminator taxa, soya (Glycine max), corn (Zea mays) potato (Solanum tuberosum), denote ingredients samples help identify source. As proof-of-concept, compact disc technology automated optical sensing RPA products. Discs worked as an analytical platform microarray format, reader/recorder detector. analysis food mixtures containing genetically modified organisms up 0.2 % showed excellent selectivity (no false-positives), reproducibility (relative error <20 %) sensitivity (0.04 ng). isothermal validated using certified reference materials successfully compared PCR-ELISA. results also confirmed it effective high-throughput tool supporting simple, low-cost safety controls, which makes ideal laboratories with limited resources.